Immunocytochemistry for Proliferating Cell Nuclear Antigen (PCNA) confirmed these data. At 2 weeks in differentiation medium, TT significantly increased mineralization in HOBs (p = 0.016) and decreased mineralization in SaOs-2 (p = 0.0007), compared to controls. Additionally, Northern blot analysis indicated a TT-induced increase in mRNA expression for Type I collagen, bone sialoprotein, and alkaline phosphatase in HOBs and a decrease of these bone markers in SaOs-2 cells. In conclusion, Therapeutic Touch appears to increase human osteoblast DNA synthesis, differentiation and mineralization, and decrease differentiation and mineralization in a human osteosarcoma-derived cell line. (c) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
J Orthop Res. 2008 Nov;26(11):1541-6. Jhaveri A, Walsh SJ, Wang Y, McCarthy M, Gronowicz G. Department of Orthopaedics, University of Connecticut Health Center, Farmington, Connecticut 06030-3105, USA.
Posted: 01/20/2009
This article provided by The International Hypnosis Research Institute.
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